Xiaorong Liu
Beijing Children’s Hospital, China
Title: Urinary CD80 is elevated in minimal change disease but not in focal segmental glomerulosclerosis
Biography
Biography: Xiaorong Liu
Abstract
Background:Early diagnose of minimal change disease(MCD)in nephrotic syndrome (NS)patients remains challenge.We often make a diagnose of MCD by invasive kidney-biopsy.CD80,is a transmembrane protein, present on podocytes in a number of experimental models of nephrotic syndrome.Urinary CD80 is significantly elevated in MCD but not in focal segmental glomerulosclerosis(FSGS)or other glomerulopathies. The aim of this study was to investigate the role of the urinary CD80 as a biomarker for diagnosis of MCD. Materials and Methods:A total of 165 subjects,129 males and 36 females were enrolled in this study. Urinary were collected from 37 patients with active MCD, 27 patients with FSGS,30 patients with other glomerulopathies and 71 healthy people. Using ELISA, compared values with the result of kidney-biopsy. Results: The concentration of urinary CD80 was significantly higher in the active MCD group (689.66±378.21ng/g creat) than those in the FSGS group (123.49±167.88ng/g creat p <0.001) ,other glomerulopathies group(152.37±220.14ng/g creat,p <0.001) and the control group(81.83±23.01 ng/g creat; p < 0.001).A cut-off value of 328.98(ng/g creat) was proposed,with an sensitivity of 81.1% and specificity of 94.4 %.The area under the ROC (receiver operating characteristic) curve for the urinary CD80 to diagnosis MCD was 0.925(95% confidence interval: 0.873-0.978）. Conclusions: This experiment has preliminarily confirmed urinary CD80 as a non-invasive diagnostic biomarker which owned a practical and significant value in the diagnosis of MCD